Superlines: Imagine Crop Innovation
“Production Lines”

Superlines Expedite GE, non-GM Variety Innovation

A Superline is a tissue culture of a crop variety integrated with GeneNeer’s cutting-edge technology. This platform allows for rapid, precise and multiplex gene editing, significantly reducing the time and money required to develop new crop varieties and refine new traits. Among the main advantages of a Superline are that, once created, one can go back to it repeatedly to add a new trait, and finished gene-edited lines can become new Superlines.

With the unique ability to completely extract the cassette, Superlines ensure complete control over trait expression levels—without leaving any genetic residue behind. This capability promotes the innovation of new GE, non-GM crop varieties.

Superlines: Rapid Gene-editing Platform

A novel transformation platform that enables multiplexing with high efficiency

 A.  Transformation and regeneration of
       Superline with GeneNeer’s technology*

B1. Calibrated Superline
B2. Superline Propagation

C.   Generating different traits

D.   Extracting the cassette 

E.   Generation of new variety with trait(s)
       inside with no trace of foreign DNA

* At any time, the original Superline of an elite variety
may be used to introduce new traits.

C   Sample Traits

  • Delayed Sprouting
  • Nematode Resistance
  • Water uptake
  • Tuber Solids
  • Nitrogen Uptake
  • Reducing Browning
  • 100% Amylopectin
  • Cold Sweetening
  • Early Blight Resistance
  • Etc.

Superlines: Rapid Gene-editing Platform

A novel transformation platform that enables multiplexing with high efficiency

C   Sample Traits

  • Delayed Sprouting
  • Nematode Resistance
  • Water uptake
  • Tuber Solids
  • Nitrogen Uptake
  • Reducing Browning
  • 100% Amylopectin
  • Cold Sweetening
  • Early Blight Resistance
  • Etc.

A. Transformation and regeneration of Superline with GeneNeer’s technology*

B1. Calibrated Superline
B2. Superline Propagation

C.   Generating different traits

D.   Extracting the cassette 

E.   Generation of new variety with trait(s) inside with no trace of foreign DNA

* At any time, the original Superline of an elite variety
may be used to introduce new traits.

The Ultimate Variety “Back-up” System

Superlines are clonally propagated and genetically stable, so they serve as a reliable reserve of elite plant material. The Superline can be propagated multiple times to test various gene editing events. Moreover, once a new trait has been added to an elite variety, a new Superline may be produced and preserved, providing the foundation for further traits to be added using gene editing at an opportune time. If the development of an enhanced variety is not successful, the scientific team can return to the elite Superline and try a new approach, without restarting the entire process.

Using this approach, new crop varieties may be innovated on a rolling basis, providing high value to the entire supply chain, from seed companies to consumers.

Revolutionizing Gene Editing, Especially in Hard-to-Breed Crops

The Superline platform offers a breakthrough approach to gene editing, particularly benefiting root and tuber crops, trees, and other vegetatively propagated plants, such as potato, banana and berries and apple trees; this is a departure from traditional breeding methods, which are slow and inefficient.

As GeneNeer’s technologies are agnostic, they may be used to develop new varieties of most crops.

A New Tool to Democratize Crop Innovation

With Superlines, the innovation of new crop varieties becomes more accessible to key players in the produce supply chain which, until now, have not had independent labs, technical expertise or sufficient funding to engage in breeding activities. Now, Tier-2 seed companies, breeders and food and beverage companies can develop varieties that best suit their local growers and customers, and/or address specific market needs.

Superlines equip breeders with the tools to:

  • Combat crop afflictions such as pests, diseases, fungi and drought.
  • Improve crop resilience without compromising plant physiology.
  • Innovate new varieties for nutrition, taste, performance, uniformity, etc.
  • Create or adapt to consumer trends with faster, non-GM trait development.

Advantages of Gene Editing Based on GeneNeer’s Superlines

1

More Competitive

Competitive companies gain a tool for rapid development and innovation of enhanced crop seeds.

2

Greater Precision and Control

Superlines facilitate greater precision and control of the gene editing process.

3

Faster

Working from Superlines at the tissue-culture level eliminates the need for crossing or single-cell assays, thereby accelerating both innovation and the duration of product development, as well as reducing the cost of crop enhancement.

4

GE, non-GM Crops

GeneNeer’s technology enables the complete extraction of the gene-editing machinery from the plant genome following editing, leaving no trace of foreign DNA and reducing the risk of off-target incidents.

5

Enabling/Facilitating

In the case of root and tuber crops, trees and other vegetatively-propagated plants, GeneNeer’s technology enables or facilitates gene editing.

6

Stackable

Multiple traits may be added to a single Superline.

7

Scalable

Superlines are agnostic and can be applied to a wide range of crops and their elite varieties.

8

Modular

Superlines can be employed in a wide range of applications, allowing for a high degree of flexibility in controlling gene expression.

9

Empowering

Providing crop growers with the right seeds helps them produce more abundant and profitable yields, while contributing to global food security.